At Scientificolab, we craft and optimize analytical methods tailored to your samples and goals—whether it’s ultra‑trace quantitation, high‑throughput screening, or complex matrix analysis. Our Method Development workflow ensures your assays are sensitive, precise, and rugged.
- Experimental Design & Feasibility
- Sample Profiling: Review sample types, concentrations, and matrices to anticipate challenges (e.g., protein binding, solvent effects).
- Technology Match: Recommend the ideal platform—HPLC, UPLC, GC, LC‑MS, or CE—based on target analytes and sensitivity needs.
- Feasibility Study: Run pilot tests to confirm detectability and evaluate potential interferences.
- Parameter Screening & Optimization
- Chromatographic Conditions
• Column chemistry, particle size, and dimensions
• Mobile‑phase composition, pH, and gradient profiles - Detector & MS Settings
• Wavelength selection, gain settings, and response factors
• MS ionization mode, collision energies, and dwell times - Flow‑through Testing
• Inject a series of standards to fine‑tune retention, resolution, and peak shape
• Use design‑of‑experiments (DoE) to systematically explore variables and interactions
- Robustness, Linearity & Sensitivity
- Robustness Checks: Deliberately vary temperature, flow rate, and pH to ensure method stability
- Calibration & Linearity: Establish calibration curves across the intended concentration range with replicate injections
- Limit of Detection (LOD) & Quantitation (LOQ): Determine the lowest levels you can reliably detect and quantify
- Validation & Documentation
- Full Validation Package
• Accuracy, precision, specificity, and carryover studies
• System suitability criteria and acceptance thresholds - Standard Operating Procedures (SOPs)
• Step‑by‑step instructions for sample prep, instrument setup, and data processing
• Troubleshooting tips and maintenance reminders - Final Report
• Detailed method description, validation data, and recommended QC checks
• Ready‑for‑audit documentation, including raw data and signed checklists
Why Choose Scientificolab?
- Expertise Across Platforms: Hands‑on experience with major brands and custom configurations
- Data‑Driven Optimization: Leverage DoE and statistical tools for efficient, reproducible results
- Audit‑Ready Documentation: Complete validation packages that satisfy regulatory and QA/QC requirements
- Turnkey Delivery: From initial concept to validated method, we handle every detail so you can start analyzing samples immediately
Ready to elevate your analytical capabilities? Contact Scientificolab today to discuss your sample challenges and let our Method Development team design the perfect protocol for your lab.
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